Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab1238)

Overview

  • Product name
    Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP)
    See all DDDDK tag primary antibodies
  • Description
    Goat polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, WB, ICCmore details
  • Immunogen

    Full length native protein (purified) corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).

  • General notes
    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000.
WB 1/1000 - 1/30000.
ICC 1/200 - 1/500.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • Anti-Flag immunostaining (using ab1238) of ntl:Flag BAC transgenic zebrafish embryos shows expression in the margin at 6 hours post fertilisation.

References

This product has been referenced in:
  • Yee A  et al. A von Willebrand factor fragment containing the D'D3 domains is sufficient to stabilize coagulation factor VIII in mice. Blood 124:445-52 (2014). Read more (PubMed: 24850761) »
  • Biffi G  et al. Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells. Nat Chem 6:75-80 (2014). ELISA . Read more (PubMed: 24345950) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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