General notesEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage buffer50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
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PurityImmunogen affinity purified
Purification notesAntibodies were affinity purified using the peptide coupled to a different carrier.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThe affinity purified antibodies were analyzed by ELISA and Western Blot analyses.
In a direct ELISA against 1 ug of ECS peptide coupled to BSA as sample, conjugate diluted 1:35,000 produced an O.D.=1.0 in a 15 minute reaction using TMB as substrate.
Optimal conjugate working dilutions should be determined for your particular assay conditions,
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
DDDDK epitope tag antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG tag antibody
References for Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab2491)
has not yet been referenced specifically in any publications.
Publishing research using ab2491? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"