Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab2491)

Overview

  • Product nameAnti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP)
    See all DDDDK tag primary antibodies
  • Description
    Chicken polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Peptide

    xxxDDDDK

    conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • General notesEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage buffer50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide coupled to a different carrier.
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesThe affinity purified antibodies were analyzed by ELISA and Western Blot analyses.
    In a direct ELISA against 1 ug of ECS peptide coupled to BSA as sample, conjugate diluted 1:35,000 produced an O.D.=1.0 in a 15 minute reaction using TMB as substrate.
    Optimal conjugate working dilutions should be determined for your particular assay conditions,
  • Target

    • RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
    • Alternative names
      • DDDDK epitope tag antibody
      • DDDK antibody
      • ddk antibody
      • DYKDDDDK antibody
      • DYKDDDDK epitope tag antibody
      • DYKDDDDK tag antibody
      • ECS epitope tag antibody
      • ECS tag antibody
      • Enterokinase Cleavage Site epitope tag antibody
      • Enterokinase Cleavage Site tag antibody
      • FLAG antibody
      • FLAG tag antibody
      see all

    References for Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab2491)

    ab2491 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"