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Our Abpromise guarantee covers the use of ab18230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 2 - 10 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Ab8517 (1/200) was used as the secondary antibody
Image part of an abreview submitted by Antibody Solutions LtdBlocked for 16 hours/ 4oC in 3.0% Non-fat milk, PBS, 0.1% Tween-20