Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243)

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Overview

  • Product name
    Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [FG4R] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Specificity
    Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
  • Tested applications
    Suitable for: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HEK293 cells transfected with DYKDDDDK
  • General notes
    If stored at 4 °C please add 0.1% sodium azide.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    ab125243 was purified by protein A affinity chromatography from mouse ascites fluid
  • Clonality
    Monoclonal
  • Clone number
    FG4R
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. (with ECL)
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In situ hybridization 1/500 - 1/2000.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) images

  • Western blot - Anti-DDDDK tag antibody [FG4R] (ab125243)
    Western blot - Anti-DDDDK tag antibody [FG4R] (ab125243)
    All lanes : Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate non-transfected
    Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector

    Developed using the ECL technique

References for Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243)

This product has been referenced in:
  • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . Read more (PubMed: 25118589) »

See 1 Publication for this product

Publishing research using ab125243? Please let us know so that we can cite the reference in this datasheet.

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