Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243)

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Overview

  • Product name
    Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [FG4R] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Host species
    Mouse
  • Specificity
    Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
  • Tested applications
    Suitable for: WB, ELISA, Dot blot, IP, In situ hybridizationmore details
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HEK293 cells transfected with DYKDDDDK
  • General notes

    If stored at 4 °C please add 0.1% sodium azide.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab125243 was purified by protein A affinity chromatography from TCS
  • Clonality
    Monoclonal
  • Clone number
    FG4R
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. (with ECL)
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In situ hybridization 1/500 - 1/2000.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • Western blot - Anti-DDDDK tag antibody [FG4R] (ab125243)
    Western blot - Anti-DDDDK tag antibody [FG4R] (ab125243)
    All lanes : Anti-DDDDK tag antibody [FG4R] - (Equivalent to FLAG antibodies from Sigma) (ab125243) at 1/1000 dilution

    Lane 1 : HEK293 cell lysate non-transfected
    Lane 2 : HEK293 cell lysate transfected with DDDDK tagged protein vector

    Developed using the ECL technique.

References

This product has been referenced in:
  • Tang S  et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB . Read more (PubMed: 25118589) »

See 1 Publication for this product

Publishing research using ab125243? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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