Anti-DDDDK tag antibody [G10] - (Equivalent to FLAG antibodies from Sigma) (ab45766)

Overview

  • Product nameAnti-DDDDK tag antibody [G10] - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [G10] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • SpecificityThis antibody recognizes recombinant proteins containing DYKDDDDK epitope tag fuse to either amino- or carboxy-terminal of the target genes. Binding is not Ca2+ dependent.
  • Tested applicationsSuitable for: WB, ELISA, IP, ICC/IFmore details
  • Species reactivity
    not applicable
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • Positive control
    • 293 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberG10
  • IsotypeIgG2b
  • Light chain typelambda
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 1 kDa.
ELISA 1/2000 - 1/5000.
IP Use at an assay dependent dilution.
ICC/IF 1/1000.

Target

  • RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

References for Anti-DDDDK tag antibody [G10] - (Equivalent to FLAG antibodies from Sigma) (ab45766)

This product has been referenced in:
  • Cao C  et al. The long intergenic noncoding RNA UFC1, a target of MicroRNA 34a, interacts with the mRNA stabilizing protein HuR to increase levels of ß-catenin in HCC cells. Gastroenterology 148:415-26.e18 (2015). Read more (PubMed: 25449213) »
  • Xu S  et al. AIF downregulation and its interaction with STK3 in renal cell carcinoma. PLoS One 9:e100824 (2014). IP . Read more (PubMed: 24992339) »

See all 3 Publications for this product

Product Wall

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I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints.
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Thank you for contacting us. The ab45766 was tested in Immunoprecipitation, it isfully guaranteed to use in IP. We haven't yet tested it in Co-IP experiments however because the principle is sameso it is more likely tobe used in Co-IP experiments also....

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Thank you for your enquiry. ab45766 is able to detect both DDDDK only peptide and fusion peptide or proteins. I hope this information helps you!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (whole cell extract prepared by TCA precipitation)
Loading amount 50000 cells
Specification whole cell extract prepared by TCA precipitation
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Carol Anderson

Verified customer

Submitted Apr 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"