Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763)

Overview

  • Product name
    Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP)
  • Conjugation
    HRP
  • Specificity
    ab49763 binds to FLAG fusion proteins and will recognize the FLAG epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal, or internal FLAG peptides)
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, ICC, EMSAmore details
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag .
    Sequence:

    DYKDDDDK

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, 150mM Sodium chloride, 10mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    M2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23497283
WB 1/100 - 1/1000.
ELISA 1/20000.
ICC 1/100 - 1/1000.
EMSA Use at an assay dependent concentration. PubMed: 22147708

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP) images

  • All lanes : Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763) at 1/3000 dilution

    Lane 1 : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).
    Lane 2 : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).
    Lane 3 : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).
    Lane 4 : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).
    Lane 5 : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).
    Lane 6 : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 45 seconds

    This image is courtesy of an anonymous Abreview

    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA for the detection of Flag-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • This image is courtesy of an anonymous Abreview

    Saccharomyces cerevisiae purified Flag-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

References for Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763)

This product has been referenced in:
  • Narni-Mancinelli E  et al. Complement factor P is a ligand for the natural killer cell-activating receptor NKp46. Sci Immunol 2:N/A (2017). Flow Cyt . Read more (PubMed: 28480349) »
  • Calì F  et al. Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway. Nucleic Acids Res 44:705-17 (2016). IP . Read more (PubMed: 26503245) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

Submitted Mar 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (NIH3T3 cells)
Loading amount
5e+006 cells
Specification
NIH3T3 cells
Treatment
10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
Gel Running Conditions
Reduced Denaturing (3-8 % Tris-Acetate Gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jan 07 2011

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Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Loading amount
2 µg
Specification
chimeric protein
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Nov 17 2010

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Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
Loading amount
125000 cells
Specification
CHO-S, transfected with plasmid containing insert
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Purified protein (Mach1 (Invitrogen))
Specification
Mach1 (Invitrogen)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Type
Direct
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Submitted Feb 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Saccharomyces cerevisiae Purified protein (BJ5453)
Loading amount
1 µg
Specification
BJ5453
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jan 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (Mach1)
Loading amount
0.01 µg
Specification
Mach1
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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