Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763)

Overview

  • Product name
    Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, ICC, EMSAmore details
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag .
    Sequence:

    DYKDDDDK

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab1238 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, 150mM Sodium chloride, 10mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    M2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23497283
WB 1/100 - 1/1000.
ELISA 1/20000.
ICC 1/100 - 1/1000.
EMSA Use at an assay dependent concentration. PubMed: 22147708

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • All lanes : Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (HRP) (ab49763) at 1/3000 dilution

    All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 45 seconds


    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA for the detection of Flag-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • Saccharomyces cerevisiae purified Flag-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

References

This product has been referenced in:
  • Narni-Mancinelli E  et al. Complement factor P is a ligand for the natural killer cell-activating receptor NKp46. Sci Immunol 2:N/A (2017). Flow Cyt . Read more (PubMed: 28480349) »
  • Shen L  et al. Combined detection of dickkopf-1 subtype classification autoantibodies as biomarkers for the diagnosis and prognosis of non-small cell lung cancer. Onco Targets Ther 10:3545-3556 (2017). Read more (PubMed: 28790847) »

See all 13 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

Submitted Mar 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (NIH3T3 cells)
Loading amount
5e+006 cells
Specification
NIH3T3 cells
Treatment
10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
Gel Running Conditions
Reduced Denaturing (3-8 % Tris-Acetate Gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

Submitted Jan 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Loading amount
2 µg
Specification
chimeric protein
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
Loading amount
125000 cells
Specification
CHO-S, transfected with plasmid containing insert
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jun 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Purified protein (Mach1 (Invitrogen))
Specification
Mach1 (Invitrogen)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Type
Direct
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Verified customer

Submitted Feb 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Saccharomyces cerevisiae Purified protein (BJ5453)
Loading amount
1 µg
Specification
BJ5453
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Jan 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (Mach1)
Loading amount
0.01 µg
Specification
Mach1
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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