Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (Phycoerythrin) (ab72469)


  • Product nameAnti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (Phycoerythrin)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Immunogen

    DYKDDDDK-Tagged peptide

  • General notes

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesSize exclusion chromatography as well as proprietary methods for purifying the final materials were used.
  • ClonalityMonoclonal
  • Clone numberM2
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab72469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (Phycoerythrin) images

  • All lanes : Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (Phycoerythrin) (ab72469) at 2 µg/ml

    Lane 1 : Hek293 cells transfected with empty vector
    Lane 2 : HEK293 cells transfected with vector overexpressing DDDDK tag

    Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution

    Performed under reducing conditions.

    Image part of an abreview submitted by Antibody Solutions Ltd

    Blocked for 16 hours/ 4oC in 3.0% Non-fat milk, PBS, 0.1% Tween-20

    See Abreview

  • Flow cytometry analysis of a recombinant (N-terminus DDDDK-tag) transmembrane cell surface receptor in CHO cells using ab72469 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

  • Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated DYKDDDDK tagged peptide and read after a 2 hour incubation at room temperature with ab72469.

References for Anti-DDDDK tag antibody [M2] - (Equivalent to FLAG antibodies from Sigma) (Phycoerythrin) (ab72469)

ab72469 has not yet been referenced specifically in any publications.

Product Wall

Data from recent batches indicates that there is approximately a 1:1 ration of PE to each antibody molecule.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells transfected with empty vector or FH-FLAG)
Loading amount 20 µg
Specification 293 cells transfected with empty vector or FH-FLAG
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

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Submitted Dec 17 2010