Anti-DDDDK tag antibody [M2] (SureLight® Allophycocyanin) (ab72569)

Overview

  • Product name
    Anti-DDDDK tag antibody [M2] (SureLight® Allophycocyanin)
    See all DDDDK tag primary antibodies
  • Description
    Mouse monoclonal [M2] to DDDDK tag - (Equivalent to FLAG antibodies from Sigma) (SureLight® Allophycocyanin)
  • Conjugation
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • Tested applications
    Suitable for: FRET, Flow Cytmore details
  • Species reactivity
    This antibody is not species specific.
  • Immunogen

    Synthetic peptide: DYKDDDDK.

  • General notes

    This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.

    Excitation maximum:652 nm Emission maximum:657.5 nm F:P 1:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, 0.1mM BSA, pH 7.4
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
  • Primary antibody notes
    This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.
  • Clonality
    Monoclonal
  • Clone number
    M2
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FRET Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

References for Anti-DDDDK tag antibody [M2] (SureLight® Allophycocyanin) (ab72569)

This product has been referenced in:
  • Kerr SA  et al. Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-Offs in New World Arenaviruses. J Virol 89:11643-53 (2015). Read more (PubMed: 26355089) »
  • Demogines A  et al. Dual host-virus arms races shape an essential housekeeping protein. PLoS Biol 11:e1001571 (2013). Flow Cyt . Read more (PubMed: 23723737) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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