Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958)

Overview

  • Product name
    Anti-DDDDK tag antibody [M2] (SureLight® P3)
    See all DDDDK tag primary antibodies
  • Description
    Mouse Monoclonal [M2] to DDDDK tag (SureLight® P3)
  • Conjugation
    SureLight® P3. Ex: 614nm, Em: 662nm
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    N/A
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag .
    Sequence:

    DYKDDDDK

  • General notes

    Excitation max. 614 nm Emission max. 662 nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
  • Clonality
    Monoclonal
  • Clone number
    M2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.
WB Use a concentration of 2 µg/ml.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Images

  • All lanes : Anti-DDDDK tag antibody [M2] (SureLight® P3) (ab72958) at 2 µg/ml

    Lane 1 : HEK293 cell line transfected with empty vector
    Lane 2 : HEK293 cell line transfected with vector overexpressing DDDDK tag

    Secondary
    Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution

    Performed under reducing conditions.

    Image part of an Abreview submitted by Antibody Solutions

    Blocking performed for 16 hours/ 4oC using 3.0% Non-fat milk, PBS, 0.1% Tween-20.

    See Abreview

References

ab72958 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells transfected with empty vector or FH-FLAG)
Loading amount
20 µg
Specification
293 cells transfected with empty vector or FH-FLAG
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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