Recombinant
RabMAb

Anti-DDIT3 antibody [EPR4943(2)] - C-terminal (ab179823)

Overview

  • Product name
    Anti-DDIT3 antibody [EPR4943(2)] - C-terminal
    See all DDIT3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4943(2)] to DDIT3 - C-terminal
  • Specificity
    Mouse and rat species are recommended based on WB results,. We do not guarantee IHC-P for mouse and rat.
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDIT3 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P35638

  • Positive control
    • Ramos, C6, Raw264.7 and HeLa cell lysates; Human DDIT3 recombinant protein; Human gastric carcinoma and pancreas tissues; permeabilized HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab179823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

We do not guarantee IHC-P for mouse and rat. 

WB 1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
    • Involvement in disease
      Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
    • Sequence similarities
      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C/EBP homologous protein antibody
      • C/EBP Homology Protein antibody
      • C/EBP zeta antibody
      • C/EBP-homologous protein 10 antibody
      • C/EBP-homologous protein antibody
      • CCAAT/enhancer binding protein homologous protein antibody
      • CEBPZ antibody
      • CHOP 10 antibody
      • CHOP antibody
      • CHOP-10 antibody
      • CHOP10 antibody
      • DDIT 3 antibody
      • DDIT-3 antibody
      • Ddit3 antibody
      • DDIT3_HUMAN antibody
      • DNA Damage Inducible Transcript 3 antibody
      • DNA damage-inducible transcript 3 protein antibody
      • GADD 153 antibody
      • GADD153 antibody
      • Growth Arrest and DNA Damage Inducible Protein 153 antibody
      • Growth arrest and DNA damage inducible protein GADD153 antibody
      • Growth arrest and DNA damage-inducible protein GADD153 antibody
      • MGC4154 antibody
      see all

    Images

    • All lanes : Anti-DDIT3 antibody [EPR4943(2)] - C-terminal (ab179823) at 20 µg

      Lane 1 : Raw 264.7 (Mouse abelson murine leukemia virus-induced tumor )whole cell lysates
      Lane 2 : NIH/3T3 (Mouse embryo) whole cell lysates
      Lane 3 : MEF (Mouse embryonic fibroblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000

      Predicted band size : 19 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST.

      The expression level in Raw 264.7 and MEF is low (PMID: 26307968 and 23546223 )

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DDIT3 using ab179823 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DDIT3 using ab179823 at 1/50 dilution.

    • Anti-DDIT3 antibody [EPR4943(2)] - C-terminal (ab179823) at 1/1000 dilution + Human DDIT3 recombinant protein at 0.01 µg

      Predicted band size : 19 kDa
    • All lanes : Anti-DDIT3 antibody [EPR4943(2)] - C-terminal (ab179823) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Raw264.7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 19 kDa

    References

    This product has been referenced in:
    • Liu Z  et al. Reducing Smad3/ATF4 was essential for Sirt1 inhibiting ER stress-induced apoptosis in mice brown adipose tissue. Oncotarget 8:9267-9279 (2017). WB . Read more (PubMed: 28030827) »
    • Allen EH  et al. Keratin 12 missense mutation induces the unfolded protein response and apoptosis in Meesmann epithelial corneal dystrophy. Hum Mol Genet 25:1176-91 (2016). IHC . Read more (PubMed: 26758872) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Sheep Cell lysate - whole cell (undifferentiated primary ovine preadipocytes)
    Gel Running Conditions
    Reduced Denaturing (10% PAG)
    Loading amount
    10 µg
    Specification
    undifferentiated primary ovine preadipocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Miss. Barbara Makela

    Verified customer

    Submitted Sep 02 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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