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Rabbit polyclonal to DDIT3 (phospho S30)
ab194533 detects endogenous level of CHOP only when phosphorylated at serine 30.
Synthetic peptide corresponding to Human DDIT3 (phospho S30) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine30(V-L-S(p)-S-D) derived from Human DDIT3 .
Database link: P35638
Jurkat cell extract, treated with PMA.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS PBS (without Mg2 +and Ca 2+)
Concentration information loading...
Immunogen affinity purified
ab194533 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
Involvement in disease
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
Belongs to the bZIP family.
Contains 1 bZIP domain.
Information by UniProt
C/EBP homologous protein antibody
C/EBP Homology Protein antibody
C/EBP zeta antibody
Western blot - Anti-DDIT3 (phospho S30) antibody (ab194533)
All lanes : Anti-DDIT3 (phospho S30) antibody (ab194533) at 1/500 dilution Lane 1 : Jurkat cell extract, treated with PMA Lane 2 : Jurkat cell extract, treated with PMA with antigen-specific peptide Predicted band size : 19 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"