Overview

  • Product name
  • Description
    Rabbit polyclonal to DDIT4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 12 amino acids from near the center of human DDIT4 (NP_061931)

  • Positive control
    • Human kidney tissue lysate; Mouse kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab106356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.

Target

  • Function
    Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
  • Tissue specificity
    Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
  • Sequence similarities
    Belongs to the DDIT4 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDIT4 antibody
    • DDIT4_HUMAN antibody
    • Dig2 antibody
    • DNA damage inducible transcript 4 antibody
    • DNA damage inducible transcript 4 protein antibody
    • DNA damage-inducible transcript 4 protein antibody
    • FLJ20500 antibody
    • HIF 1 responsive protein RTP801 antibody
    • HIF 1 responsive RTP801 antibody
    • HIF-1 responsive protein RTP801 antibody
    • Protein regulated in development and DNA damage response 1 antibody
    • REDD-1 antibody
    • REDD1 antibody
    • RTP801 antibody
    see all

Images

  • Lane 1 : Anti-DDIT4 antibody (ab106356) at 0.5 µg/ml
    Lane 2 : Anti-DDIT4 antibody (ab106356) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 25 kDa
  • Immunohistochemistry detection of DDIT4 in paraffin embedded mouse kidney tissue section using ab106356 at 5 µg/ml.

References

This product has been referenced in:
  • Gupta I  et al. Ascorbate attenuates pulmonary emphysema by inhibiting tobacco smoke and Rtp801-triggered lung protein modification and proteolysis. Proc Natl Acad Sci U S A 113:E4208-17 (2016). Read more (PubMed: 27382160) »
  • Zhao H  et al. DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27898044) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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