Overview

  • Product name
  • Description
    Rabbit polyclonal to DDIT4L
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: KLGCSKVLVP EKLTQRIAQD VLRLSSTEPC GLRGCVMHVN LEIENVCKKL , corresponding to internal sequence amino acids 72-121 of Human DDIT4L (NP_660287).

  • Positive control
    • 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    DDIT4L inhibits cell growth by regulating the FRAP1 pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. It is up regulated in atherosclerotic plaques relative to healthy segments of the same artery.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • DNA damage inducible transcript 4 like antibody
    • DNA damage inducible transcript 4 like protein antibody
    • HIF 1 responsive protein RTP801 like antibody
    • Homolog of mouse SMHS1 antibody
    • Protein regulated in development and DNA damage response 2 antibody
    • Protein regulated in development and DNA damage responses 2 antibody
    • REDD 2 antibody
    • REDD2 antibody
    • Regulated in development and DNA damage response 2 antibody
    • RTP801L antibody
    see all

Anti-DDIT4L antibody images

  • Anti-DDIT4L antibody (ab84562) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DDIT4L antibody (ab84562)

ab84562 has not yet been referenced specifically in any publications.

Product Wall

According to our records, ab84562 was proving difficult to use in WB with mouse samples and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting to hear back in order to resolve this...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
I would like to point out that mouse samples have not been tried with this antibody, and we therefore do not know...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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