Overview

  • Product name
  • Description
    Rabbit polyclonal to DDRGK1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 of Human DDRGK1 (NP_076424).

  • Positive control
    • MCF7 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Protein which interacts with the E3 UFM1-protein ligase UFL1 and one of its substrates TRIP4 and is required for TRIP4 ufmylation. Through TRIP4 ufmylation may regulate nuclear receptors-mediated transcription (PubMed:25219498). May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIB, the inhibitor of NF-kappa-B (PubMed:23675531). May also play a role in the cellular response to endoplasmic reticulum stress.
  • Tissue specificity
    Widely expressed (at protein level). In the brain, highest levels in medulla oblongata, followed by cerebral cortex, cerebellum and frontal lobe.
  • Sequence similarities
    Belongs to the DDRGK1 family.
    Contains 1 PCI domain.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination probably triggers proteasomal degradation and is negatively regulated by UFL1, the enzyme involved in the ufmylation of DDRGK1.
    Ufmylated. Conjugated to ubiquitin-like protein UFM1, probably at Lys-267 by UFL1.
  • Cellular localization
    Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110001I20Rik antibody
    • 2600009E05Rik antibody
    • C20orf116 antibody
    • Chromosome 20 open reading frame 116 antibody
    • Dashurin antibody
    • DDRGK domain containing 1 antibody
    • DDRGK domain containing protein 1 antibody
    • DDRGK domain-containing protein 1 antibody
    • DDRGK_HUMAN antibody
    • Ddrgk1 antibody
    • dJ1187M17.3 antibody
    • MGC2592 antibody
    • OTTHUMP00000030088 antibody
    • RGD1309979 antibody
    • RP23-100C5.9 antibody
    • UFM1-binding and PCI domain-containing protein 1 antibody
    see all

Images

  • Anti-DDRGK1 antibody (ab99121) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Predicted band size : 36 kDa

References

ab99121 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99121.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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