Anti-DDT antibody (ab115785)

Overview

  • Product name
  • Description
    Rabbit polyclonal to DDT
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a sequence at the C-terminal of Human DDT.

  • Positive control
    • Human placenta tissue; Rat brain, pancreas, liver and lung tissue lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 0.45% Sodium chloride, 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

Anti-DDT antibody images

  • ICC/IF image of ab115785 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115785 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-DDT antibody (ab115785) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat pancreas tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat lung tissue lysate


    Predicted band size : 13 kDa
  • ab115785 at 1µg/ml staining DTT in Paraffin-embedded Human placenta tissue by Immunohistochemistry.

References for Anti-DDT antibody (ab115785)

ab115785 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - other (rat or mouse heart soluble fraction)
Loading amount
100 µg
Specification
rat or mouse heart soluble fraction
Gel Running Conditions
Reduced Denaturing (18%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jan 17 2013

Thank you for your inquiry.

I heard back from the lab that they used the C-terminal sequence of DDT as the immunogen. There is no cross-reactivity expected with mouse MIF according to a BLAST alignment.


To our knowledge, ab115...

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free of charge replacement arranged.

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees...

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Application
Immunoprecipitation
Sample
Mouse Tissue lysate - other (liver cytosol)
Total protein in input
500 µg
Specification
liver cytosol
Immuno-precipitation step
Other - Dynabeads bound to Protein A
Username

Abcam user community

Verified customer

Submitted Mar 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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