• Product name
  • Description
    Rabbit polyclonal to DDX1
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 650 and the C-terminus (residue 697) of human DDX1. (AAH12739.1)

  • Positive control
    • Whole cell lysate from Hela cells


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 3-5 µg/mg of lysate.


  • Function
    Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.
  • Tissue specificity
    Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX1 subfamily.
    Contains 1 B30.2/SPRY domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The helicase domain is involved in the stimulation of RELA transcriptional activity.
  • Post-translational
    Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase DDX1 antibody
    • ATP-dependent RNA helicase DDX1 antibody
    • DBP RB antibody
    • DBP-RB antibody
    • DDX1 antibody
    • DDX1_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody
    • DEAD box 1 antibody
    • DEAD box polypeptide 1 antibody
    • DEAD box protein 1 antibody
    • DEAD box protein retinoblastoma antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody
    see all

Anti-DDX1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation/ Western Blot of DDX1
    HeLa whole cell lysate, (1 mg for IP, 1/4 of IP loaded) with ab70252 at 0.03µg/ml for WB.
    Lane 1: ab70251 at 3µg/mg lysate for IP.
    Lane 2: Control IgG.

    Detection: Chemiluminescence with exposure time of 1 minute.
  • All lanes : Anti-DDX1 antibody (ab70252) at 0.03 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 20 µg
    Lane 2 : Whole cell lysate from Hela cells at 40 µg
    Lane 3 : Whole cell lysate from Hela cells at 80 µg

    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DDX1 antibody (ab70252)

ab70252 has not yet been referenced specifically in any publications.

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