• Product nameAnti-DDX1 antibody
    See all DDX1 primary antibodies
  • Description
    Mouse monoclonal to DDX1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 642-741 of Human DDX1 with proprietary tag (NP_004930)

  • Positive control
    • Hela nuclear extract


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab77213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 10ng/ml as a capture antibody when used against the immunogen.


  • FunctionActs as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.
  • Tissue specificityHighest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX1 subfamily.
    Contains 1 B30.2/SPRY domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe helicase domain is involved in the stimulation of RELA transcriptional activity.
  • Post-translational
    Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase DDX1 antibody
    • ATP-dependent RNA helicase DDX1 antibody
    • DBP RB antibody
    • DBP-RB antibody
    • DDX1 antibody
    • DDX1_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody
    • DEAD box 1 antibody
    • DEAD box polypeptide 1 antibody
    • DEAD box protein 1 antibody
    • DEAD box protein retinoblastoma antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody
    see all

Anti-DDX1 antibody images

  • Anti-DDX1 antibody (ab77213) at 5 µg/ml + Recombinant fragment of human DDX1 at 0.2 µg

    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 82 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-DDX1 antibody (ab77213) at 5 µg/ml + HeLa nuclear lysate at 25 µg

    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 82 kDa
    Observed band size : 82 kDa

References for Anti-DDX1 antibody (ab77213)

ab77213 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77213.
Please use the links above to contact us or submit feedback about this product.