• Product nameAnti-DDX1 antibody
    See all DDX1 primary antibodies
  • Description
    Rabbit polyclonal to DDX1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (ILGGGDVLMA AETGSGKTGA FSIPVIQIVY ETLKDQQEGK KGKTTIKTGA) of human DDX1 (NP_004930).

  • Positive control
    • Human fetal muscle cytoplasmic or nuclear lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab83275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionActs as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs.
  • Tissue specificityHighest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX1 subfamily.
    Contains 1 B30.2/SPRY domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe helicase domain is involved in the stimulation of RELA transcriptional activity.
  • Post-translational
    Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase DDX1 antibody
    • ATP-dependent RNA helicase DDX1 antibody
    • DBP RB antibody
    • DBP-RB antibody
    • DDX1 antibody
    • DDX1_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 1 antibody
    • DEAD box 1 antibody
    • DEAD box polypeptide 1 antibody
    • DEAD box protein 1 antibody
    • DEAD box protein retinoblastoma antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1 antibody
    see all

Anti-DDX1 antibody images

  • Anti-DDX1 antibody (ab83275) at 1 µg/ml + fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DDX1 antibody (ab83275)

ab83275 has not yet been referenced specifically in any publications.

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