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Mouse monoclonal [2248C2a] to DDX17
Recombinant fragment of human DDX17
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
Concentration information loading...
Protein G purified
ab71958 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 33 kDa (predicted molecular weight: 72 kDa).
Use at an assay dependent dilution.
RNA-dependent ATPase activity.
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
DEAD (Asp Glu Ala Asp) box helicase 17 antibody
Western blot - Anti-DDX17 antibody [2248C2a] (ab71958)
Anti-DDX17 antibody [2248C2a] (ab71958) + immunising recombinant protein
Predicted band size:
Observed band size:
33 kDa (
why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"