Anti-DDX17 antibody (ab24601)
Key features and details
- Rabbit polyclonal to DDX17
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DDX17 antibody
See all DDX17 primary antibodies -
Description
Rabbit polyclonal to DDX17 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa).
This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data). |
Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa). This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data). |
Target
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Function
RNA-dependent ATPase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10521 Human
- Entrez Gene: 67040 Mouse
- Entrez Gene: 315133 Rat
- Omim: 608469 Human
- SwissProt: Q92841 Human
- SwissProt: Q501J6 Mouse
- Unigene: 528305 Human
- Unigene: 725500 Human
see all -
Alternative names
- Ddx17 antibody
- DDX17_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box helicase 17 antibody
see all
Images
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All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : DDX17 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 80,73 kDaLanes 1 - 4: Merged signal (red and green). Green - ab24601 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab24601 was shown to recognize DDX17 in wild-type HEK 293 cells as signal was lost at the expected MW in DDX17 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and DDX17 knockout samples were subjected to SDS-PAGE. Ab24601 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80,73 kDa
Observed band size: 73,80 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab24601 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab24601 has been referenced in 13 publications.
- Shallak M et al. The endogenous HBZ interactome in ATL leukemic cells reveals an unprecedented complexity of host interacting partners involved in RNA splicing. Front Immunol 13:939863 (2022). PubMed: 35979358
- Terrone S et al. RNA helicase-dependent gene looping impacts messenger RNA processing. Nucleic Acids Res 50:9226-9246 (2022). PubMed: 36039747
- Ameur LB et al. Intragenic recruitment of NF-?B drives splicing modifications upon activation by the oncogene Tax of HTLV-1. Nat Commun 11:3045 (2020). PubMed: 32546717
- Miro J et al. First Identification of RNA-Binding Proteins That Regulate Alternative Exons in the Dystrophin Gene. Int J Mol Sci 21:N/A (2020). PubMed: 33096920
- Lee SH & Mayr C Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation. Mol Cell 74:701-712.e9 (2019). PubMed: 30948266