Overview

  • Product nameAnti-DDX17 antibody
    See all DDX17 primary antibodies
  • Description
    Rabbit polyclonal to DDX17
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human DDX17.

    (Peptide available as ab31640.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate;

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23223531
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 80,73 kDa (predicted molecular weight: 80,73 kDa).

This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (80 kDa) and isoform 2 (73 kDa) of Human DDX17(SwissProt data).

Target

  • FunctionRNA-dependent ATPase activity.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ddx17 antibody
    • DDX17_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 17 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 17 antibody
    • DEAD box helicase 17 antibody
    • DEAD box protein 17 antibody
    • DEAD box protein p72 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 17 antibody
    • P72 antibody
    • Probable ATP-dependent RNA helicase DDX17 antibody
    • RH70 antibody
    • RNA dependent helicase p72 antibody
    • RNA-dependent helicase p72 antibody
    see all

Anti-DDX17 antibody images

  • All lanes : Anti-DDX17 antibody (ab24601) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80,73 kDa
    Observed band size : 73,80 kDa
    Additional bands at : 14 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab24601 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-DDX17 antibody (ab24601)

This product has been referenced in:
  • Samaan S  et al. The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24275493) »
  • Germann S  et al. Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor. Oncogene : (2012). Read more (PubMed: 22266867) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"