Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX17
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 550 and 613 of human DDX17

  • Positive control
    • Whole cell lysate from HeLa, 293T, MCF7 and K562 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70184 was affinity purified using an epitope specific to DDX17 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/15000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

Target

  • Function
    RNA-dependent ATPase activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ddx17 antibody
    • DDX17_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 17 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 17 antibody
    • DEAD box helicase 17 antibody
    • DEAD box protein 17 antibody
    • DEAD box protein p72 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 17 antibody
    • P72 antibody
    • Probable ATP-dependent RNA helicase DDX17 antibody
    • RH70 antibody
    • RNA dependent helicase p72 antibody
    • RNA-dependent helicase p72 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DDX17 with ab70184 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX17 antibody (ab70184) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : MCF7 whole cell lysate at 50 µg
    Lane 6 : K562 whole cell lysate at 50 µg


    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References

This product has been referenced in:
  • Motiño O  et al. Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver Is DEAD-Box Helicase p68 (DDX5) Dependent: Role in Insulin Signaling. Mol Cell Biol 35:2554-67 (2015). WB . Read more (PubMed: 25963660) »
  • Zaccara S  et al. p53-directed translational control can shape and expand the universe of p53 target genes. Cell Death Differ N/A:N/A (2014). Read more (PubMed: 24926617) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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