Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX18
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    A region between residues 1 and 50 of human DDX18 (NP_006764.3)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 1 - 4 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probable RNA-dependent helicase.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX18 antibody
      • ATP-dependent RNA helicase DDX18 antibody
      • DDX 18 antibody
      • DDX18 antibody
      • DDX18_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 18 antibody
      • DEAD box polypeptide 18 antibody
      • DEAD box protein 18 antibody
      • FLJ33908 antibody
      • MrDb antibody
      • Myc regulated DEAD box protein antibody
      • Myc-regulated DEAD box protein antibody
      see all

    Anti-DDX18 antibody images

    • All lanes : Anti-DDX18 antibody (ab70526) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 75 kDa
      Observed band size : 75 kDa


      Exposure time : 3 minutes
    • Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70526 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.

    References for Anti-DDX18 antibody (ab70526)

    ab70526 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70526.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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