Overview

  • Product nameAnti-DDX18 antibody
    See all DDX18 primary antibodies
  • Description
    Rabbit polyclonal to DDX18
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Orangutan
  • Immunogen

    A region between residue 625 and the C terminus (residue 670) of human DDX18 (NP_006764.3)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 1 - 4 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbable RNA-dependent helicase.
    • Sequence similaritiesBelongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX18 antibody
      • ATP-dependent RNA helicase DDX18 antibody
      • DDX 18 antibody
      • DDX18 antibody
      • DDX18_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 18 antibody
      • DEAD box polypeptide 18 antibody
      • DEAD box protein 18 antibody
      • FLJ33908 antibody
      • MrDb antibody
      • Myc regulated DEAD box protein antibody
      • Myc-regulated DEAD box protein antibody
      see all

    Anti-DDX18 antibody images

    • All lanes : Anti-DDX18 antibody (ab70527) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 75 kDa
      Observed band size : 75 kDa


      Exposure time : 3 minutes
    • Detection of DDX18 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70526 (Lane 1) and ab70527 (Lane 2) at 3 µg/mg lysate for IP. ab70527 was used at 1 µg/ml for subsequent WB detection. Lane 3 represents control IgG IP.
      Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-DDX18 antibody (ab70527)

    ab70527 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70527.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"