Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX19B
  • Specificity
    The immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human DDX19B. (NP_009173.1.)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ATP-dependent RNA helicase involved in mRNA export from the nucleus.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization
      Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP-dependent RNA helicase DDX19B antibody
      • DBP5 antibody
      • Dbp5, yeast, homolog of antibody
      • DD19B_HUMAN antibody
      • DDX19 antibody
      • DDX19B antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B antibody
      • DEAD box protein 19B antibody
      • DEAD box RNA helicase DEAD5 antibody
      • DEAD-box protein 5 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog) antibody
      • DEAD/H BOX 19 antibody
      • helicase DEAD5 antibody
      • RNAh antibody
      • TDBP antibody
      • yeast Dbp5 homolog antibody
      see all

    Images

    • All lanes : Anti-DDX19B antibody (ab70305) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 53 kDa
      Observed band size : 53 kDa


      Exposure time : 30 seconds

    References

    ab70305 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70305.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up