Overview

  • Product nameAnti-DDX19B antibody
    See all DDX19B primary antibodies
  • Description
    Rabbit polyclonal to DDX19B
  • SpecificityThe immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human DDX19B. (NP_009173.1.)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionATP-dependent RNA helicase involved in mRNA export from the nucleus.
    • Sequence similaritiesBelongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localizationCytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP-dependent RNA helicase DDX19B antibody
      • DBP5 antibody
      • Dbp5, yeast, homolog of antibody
      • DD19B_HUMAN antibody
      • DDX19 antibody
      • DDX19B antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B antibody
      • DEAD box protein 19B antibody
      • DEAD box RNA helicase DEAD5 antibody
      • DEAD-box protein 5 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog) antibody
      • DEAD/H BOX 19 antibody
      • helicase DEAD5 antibody
      • RNAh antibody
      • TDBP antibody
      • yeast Dbp5 homolog antibody
      see all

    Anti-DDX19B antibody images

    • All lanes : Anti-DDX19B antibody (ab70305) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 53 kDa
      Observed band size : 53 kDa


      Exposure time : 30 seconds

    References for Anti-DDX19B antibody (ab70305)

    ab70305 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70305.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"