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Rabbit polyclonal to DDX19B
The immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity with this homolog has not been assessed.
Predicted to work with:
Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residues 400 and 450 of human DDX19B. (NP_009173.1)
HeLa, 293T and NIH3T3 whole cell lystes.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
Use at 2 µg/mg of lysate.
ATP-dependent RNA helicase involved in mRNA export from the nucleus.
Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Cytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
Information by UniProt
ATP-dependent RNA helicase DDX19B antibody
Dbp5, yeast, homolog of antibody
Western blot - DDX19B antibody (ab70306)
All lanes :
Anti-DDX19B antibody (ab70306) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Lane 5 :
NIH3T3 whole cell lysate at 50 µg
Predicted band size:
Observed band size:
60 kDa (
why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"