Overview

  • Product nameAnti-DDX19B antibody
    See all DDX19B primary antibodies
  • Description
    Rabbit polyclonal to DDX19B
  • SpecificityThe immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity with this homolog has not been assessed.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residues 400 and 450 of human DDX19B. (NP_009173.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lystes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IP Use at 2 µg/mg of lysate.

Target

  • FunctionATP-dependent RNA helicase involved in mRNA export from the nucleus.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX19/DBP5 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationCytoplasm. Nucleus > nuclear pore complex. Nucleus membrane. Nuclear pore complex cytoplasmic fibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP-dependent RNA helicase DDX19B antibody
    • DBP5 antibody
    • Dbp5, yeast, homolog of antibody
    • DD19B_HUMAN antibody
    • DDX19 antibody
    • DDX19B antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B antibody
    • DEAD box protein 19B antibody
    • DEAD box RNA helicase DEAD5 antibody
    • DEAD-box protein 5 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog) antibody
    • DEAD/H BOX 19 antibody
    • helicase DEAD5 antibody
    • RNAh antibody
    • TDBP antibody
    • yeast Dbp5 homolog antibody
    see all

Anti-DDX19B antibody images

  • All lanes : Anti-DDX19B antibody (ab70306) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

References for Anti-DDX19B antibody (ab70306)

ab70306 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Mouse Cell lysate - whole cell (Mouse pancreatic cancer)
Specification Mouse pancreatic cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Feb 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 15 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"