Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX21
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 725 and the C-terminal (residue 783) of Human DDX21 (NP_004719.2)

  • Positive control
    • Human larynx squamous cell carcinoma. Mouse squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
    The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ddx21 antibody
    • DDX21_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody
    • DEAD box protein 21 antibody
    • EC 3.6.1.- antibody
    • Gu alpha antibody
    • Gu protein antibody
    • Gu-alpha antibody
    • GUA antibody
    • GURDB antibody
    • Nucleolar RNA helicase 2 antibody
    • Nucleolar RNA helicase Gu antibody
    • Nucleolar RNA helicase II antibody
    • RH II/Gu antibody
    • RH II/GuA antibody
    • RNA helicase II/Gu alpha antibody
    see all

Images

  • Detection of Human DDX21 by Immunohistochemistry.
    Sample: FFPE section of Human larynx squamous cell carcinoma.
    Antibody: ab84394 used at a dilution of 1/250.
    Detection: DAB.
  • Detection of Mouse DDX21 by Immunohistochemistry.
    Sample: FFPE section of Mouse squamous cell carcinoma.
    Antibody: ab84394 used at a dilution of 1/250.
    Detection: DAB.

References

This product has been referenced in:
  • Lund RR  et al. NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics 14:2988-99 (2015). IHC-P ; Human . Read more (PubMed: 26351264) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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