The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionCan unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX21/DDX50 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
DomainThe two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus. The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
Detection of Mouse DDX21 by Immunohistochemistry. Sample: FFPE section of Mouse squamous cell carcinoma.Antibody: ab84394 used at a dilution of 1/250. Detection: DAB.
References for Anti-DDX21 antibody (ab84394)
This product has been referenced in:
Lund RR et al. NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics14:2988-99 (2015).
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