• Product name
  • Description
    Rabbit polyclonal to DDX21
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 725 and the C-terminal (residue 783) of Human DDX21 (NP_004719.2)

  • Positive control
    • Human larynx squamous cell carcinoma. Mouse squamous cell carcinoma.



Our Abpromise guarantee covers the use of ab84394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
    The 3 X 5 AA repeats seem to be critical for the RNA folding activity.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ddx21 antibody
    • DDX21_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody
    • DEAD box protein 21 antibody
    • EC 3.6.1.- antibody
    • Gu alpha antibody
    • Gu protein antibody
    • Gu-alpha antibody
    • GUA antibody
    • GURDB antibody
    • Nucleolar RNA helicase 2 antibody
    • Nucleolar RNA helicase Gu antibody
    • Nucleolar RNA helicase II antibody
    • RH II/Gu antibody
    • RH II/GuA antibody
    • RNA helicase II/Gu alpha antibody
    see all

Anti-DDX21 antibody images

  • Detection of Human DDX21 by Immunohistochemistry.
    Sample: FFPE section of Human larynx squamous cell carcinoma.
    Antibody: ab84394 used at a dilution of 1/250.
    Detection: DAB.
  • Detection of Mouse DDX21 by Immunohistochemistry.
    Sample: FFPE section of Mouse squamous cell carcinoma.
    Antibody: ab84394 used at a dilution of 1/250.
    Detection: DAB.

References for Anti-DDX21 antibody (ab84394)

This product has been referenced in:
  • Lund RR  et al. NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics 14:2988-99 (2015). IHC-P ; Human . Read more (PubMed: 26351264) »

See 1 Publication for this product

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