Overview

  • Product nameAnti-DDX23 antibody
    See all DDX23 primary antibodies
  • Description
    Rabbit polyclonal to DDX23
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human DDX23 (NP_004809.2).

  • Positive control
    • Breast Carcinoma, Non-Small Cell Lung Cancer, Stomach Adenocarcinom

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab96254 was affinity purified using an epitope specific to DDX23 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended, with tris-EDTA pH 9.0 buffer to enhance staining. Likely to work with frozen sections.

Target

  • FunctionInvolved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX23/PRP28 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa U5 snRNP-specific protein antibody
    • DDX23 antibody
    • DDX23_HUMAN antibody
    • DEAD box protein 23 antibody
    • EC 3.6.1.- antibody
    • Probable ATP-dependent RNA helicase DDX23 antibody
    • PRP28 homolog antibody
    • U5-100kD antibody
    see all

Anti-DDX23 antibody images

  • ab96254, at 1/250 dilution, staining DDX23 in formalin-fixed, paraffin-embedded Human breast carcinoma by Immunohistochemistry using DAB detection. Epitope retrieval was performed.

References for Anti-DDX23 antibody (ab96254)

ab96254 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"