The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Epitope exposure is recommended, with tris-EDTA pH 9.0 buffer to enhance staining.
Likely to work with frozen sections.
FunctionInvolved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX23/PRP28 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Post-translational modificationsIn vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation.