Overview

  • Product nameAnti-DDX24 antibody
    See all DDX24 primary antibodies
  • Description
    Mouse polyclonal to DDX24
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human DDX24 protein (AAH96826.1, 804 a.a).

  • Positive control
    • human liver lysate, DDX24 transfected 293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 100, 150 kDa (predicted molecular weight: 95 kDa).

Target

  • FunctionATP-dependent RNA helicase.
  • Tissue specificityUbiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX24 antibody
    • ATP-dependent RNA helicase ddx24 antibody
    • DDX 24 antibody
    • ddx24 antibody
    • DDX24_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
    • DEAD box protein 24 antibody
    • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
    • S. cerevisiae CHL1 like helicase antibody
    see all

Anti-DDX24 antibody images

  • Anti-DDX24 antibody (ab69148) at 1/500 dilution + human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-DDX24 antibody (ab69148) at 1/500 dilution

    Lane 1 : DDX24 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

References for Anti-DDX24 antibody (ab69148)

ab69148 has not yet been referenced specifically in any publications.

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