- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-DDX24 antibody
See all DDX24 primary antibodies -
DescriptionRabbit polyclonal to DDX24
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of Human DDX24 (NP_065147.1)
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Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab70462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionATP-dependent RNA helicase.
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Tissue specificityUbiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
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Sequence similaritiesBelongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 57062 Human
- Omim: 606181 Human
- SwissProt: Q9GZR7 Human
- Unigene: 510328 Human
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Alternative names
- ATP dependent RNA helicase DDX24 antibody
- ATP-dependent RNA helicase ddx24 antibody
- DDX 24 antibody
see all
Images
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Western blot - DDX24 antibody (ab70462)All lanes : Anti-DDX24 antibody (ab70462) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 seconds
References
ab70462 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"