Overview

  • Product nameAnti-DDX24 antibody
    See all DDX24 primary antibodies
  • Description
    Rabbit polyclonal to DDX24
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of Human DDX24 (NP_065147.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionATP-dependent RNA helicase.
    • Tissue specificityUbiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
    • Sequence similaritiesBelongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX24 antibody
      • ATP-dependent RNA helicase ddx24 antibody
      • DDX 24 antibody
      • ddx24 antibody
      • DDX24_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
      • DEAD box protein 24 antibody
      • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
      • S. cerevisiae CHL1 like helicase antibody
      see all

    Anti-DDX24 antibody images

    • All lanes : Anti-DDX24 antibody (ab70462) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 97 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 seconds

    References for Anti-DDX24 antibody (ab70462)

    ab70462 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 31 2014

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Total protein in input 250 µg
    Username

    Abcam user community

    Verified customer

    Submitted Jan 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"