Customer reviews and FAQs

Overview

  • Product name
    Anti-DDX24 antibody
    See all DDX24 primary antibodies
  • Description
    Rabbit polyclonal to DDX24
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of Human DDX24 (NP_065147.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      ATP-dependent RNA helicase.
    • Tissue specificity
      Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
      • Alternative names
        • ATP dependent RNA helicase DDX24 antibody
        • ATP-dependent RNA helicase ddx24 antibody
        • DDX 24 antibody
        • ddx24 antibody
        • DDX24_HUMAN antibody
        • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
        • DEAD box protein 24 antibody
        • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
        • S. cerevisiae CHL1 like helicase antibody
        see all

      Anti-DDX24 antibody images

      • Western blot - DDX24 antibody (ab70462)
        Western blot - DDX24 antibody (ab70462)
        All lanes : Anti-DDX24 antibody (ab70462) at 0.04 µg/ml

        Lane 1 : HeLa whole cell lysate at 50 µg
        Lane 2 : HeLa whole cell lysate at 15 µg
        Lane 3 : HeLa whole cell lysate at 5 µg
        Lane 4 : 293T whole cell lysate at 50 µg
        Lane 5 : NIH3T3 whole cell lysate at 50 µg


        Predicted band size : 97 kDa
        Observed band size : 120 kDa (why is the actual band size different from the predicted?)


        Exposure time : 3 seconds

      References for Anti-DDX24 antibody (ab70462)

      ab70462 has not yet been referenced specifically in any publications.

      Publishing research using ab70462? Please let us know so that we can cite the reference in this datasheet.

      Product Wall

      Submit an abreview for ab70462 Submit a question for ab70462

      2 Abreviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-DDX24 antibody

       Average
      Abreviews
      Application
      Western blot
      Loading amount
      50 µg
      Gel Running Conditions
      Reduced Denaturing (10%)
      Sample
      Human Cell lysate - whole cell (HEK293)
      Specification
      HEK293
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Jan 31 2014

      Immunoprecipitation abreview for Anti-DDX24 antibody

       Good
      Abreviews
      Application
      Immunoprecipitation
      Immuno-precipitation step
      Protein G
      Sample
      Human Cell lysate - whole cell (HEK293)
      Specification
      HEK293
      Total protein in input
      250 µg
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Jan 31 2014

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up