Anti-DDX24 antibody (ab70462)
Key features and details
- Rabbit polyclonal to DDX24
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX24 antibody
See all DDX24 primary antibodies -
Description
Rabbit polyclonal to DDX24 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of Human DDX24 (NP_065147.1)
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Positive control
- HeLa, 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
ATP-dependent RNA helicase. -
Tissue specificity
Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine. -
Sequence similarities
Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 57062 Human
- Omim: 606181 Human
- SwissProt: Q9GZR7 Human
- Unigene: 510328 Human
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Alternative names
- ATP dependent RNA helicase DDX24 antibody
- ATP-dependent RNA helicase ddx24 antibody
- DDX 24 antibody
see all
Images
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All lanes : Anti-DDX24 antibody (ab70462) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab70462 has not yet been referenced specifically in any publications.