Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX24
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 325 and 375 of Human DDX24 (NP_065147.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ATP-dependent RNA helicase.
    • Tissue specificity
      Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX24 antibody
      • ATP-dependent RNA helicase ddx24 antibody
      • DDX 24 antibody
      • ddx24 antibody
      • DDX24_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 24 antibody
      • DEAD box protein 24 antibody
      • DEAD/H (Asp Glu Ala Asp) box polypeptide 24 antibody
      • S. cerevisiae CHL1 like helicase antibody
      see all

    Images

    • All lanes : Anti-DDX24 antibody (ab70463) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 97 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 160 kDa,230 kDa,350 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 seconds

    References

    ab70463 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70463.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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