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Rabbit polyclonal to DDX24
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 325 and 375 of Human DDX24 (NP_065147.1)
HeLa, 293T and NIH3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ATP-dependent RNA helicase.
Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
ATP dependent RNA helicase DDX24 antibody
ATP-dependent RNA helicase ddx24 antibody
DDX 24 antibody
Western blot - DDX24 antibody (ab70463)
All lanes :
Anti-DDX24 antibody (ab70463) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Lane 5 :
NIH3T3 whole cell lysate at 50 µg
Predicted band size :
Observed band size :
120 kDa (
why is the actual band size different from the predicted?
Additional bands at :
160 kDa,230 kDa,350 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"