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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function ATP-dependent RNA helicase.
Tissue specificity Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
Sequence similarities Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
ATP dependent RNA helicase DDX24 antibody
ATP-dependent RNA helicase ddx24 antibody
DDX 24 antibody
Anti-DDX24 antibody images
Western blot - DDX24 antibody (ab70463)
All lanes : Anti-DDX24 antibody (ab70463) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg Predicted band size : 97 kDa Observed band size : 120 kDa ( why is the actual band size different from the predicted?) Additional bands at : 160 kDa,230 kDa,350 kDa. We are unsure as to the identity of these extra bands. Exposure time : 10 seconds
References for Anti-DDX24 antibody (ab70463)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"