Overview

  • Product name
    Anti-DDX27 antibody [2251C2a]
    See all DDX27 primary antibodies
  • Description
    Mouse monoclonal [2251C2a] to DDX27
  • Tested applications
    Suitable for: WB, Dot blot, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human DDX27

  • Positive control
    • HeLa cells and HeLa and NIH3T3 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab50696 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2251C2a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 90 kDa.
Dot blot Use at an assay dependent dilution.
ICC 1/5.

Target

  • Function
    Probable ATP-dependent RNA helicase.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C86129 antibody
    • cb843 antibody
    • DDX27 antibody
    • DDX27_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody
    • DEAD box protein 27 antibody
    • Deficiency of ribosomal subunits protein 1 homolog antibody
    • dJ686N3.1 antibody
    • DKFZp667N057 antibody
    • DRS1 antibody
    • FLJ12917 antibody
    • FLJ20596 antibody
    • FLJ22238 antibody
    • HSPC259 antibody
    • MGC1018 antibody
    • MGC163147 antibody
    • PP3241 antibody
    • Probable ATP-dependent RNA helicase DDX27 antibody
    • RHLP antibody
    • RNA helicase like protein antibody
    • Rrp3p antibody
    see all

Images

  • Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • Anti-DDX27 antibody [2251C2a] (ab50696) at 1/50 dilution + NIH3T3 whole cell lyaste at 25 µg

    Secondary
    Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • ab50696 at 1/5 dilution staining human DDX27 in HeLa cells by Immunocytochemistry. A 1/200 dilution of AlexaFluor488 Goat Anti-mouse IgG was used as secondary.

References

ab50696 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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