Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX27
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: KKKRAATTLD EKIEKVRKKR KTEDKEAKSG KLEKEKEAKE GSEPKEQEDL, corresponding to N terminal amino acids 109-158 of Human DDX27

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 88 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Probable ATP-dependent RNA helicase.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C86129 antibody
    • cb843 antibody
    • DDX27 antibody
    • DDX27_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody
    • DEAD box protein 27 antibody
    • Deficiency of ribosomal subunits protein 1 homolog antibody
    • dJ686N3.1 antibody
    • DKFZp667N057 antibody
    • DRS1 antibody
    • FLJ12917 antibody
    • FLJ20596 antibody
    • FLJ22238 antibody
    • HSPC259 antibody
    • MGC1018 antibody
    • MGC163147 antibody
    • PP3241 antibody
    • Probable ATP-dependent RNA helicase DDX27 antibody
    • RHLP antibody
    • RNA helicase like protein antibody
    • Rrp3p antibody
    see all

Anti-DDX27 antibody images

  • Anti-DDX27 antibody (ab36312) at 0.625 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 88 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-DDX27 antibody (ab36312)

ab36312 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab36312.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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