Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX27
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within residues 750-796 of Human DDX27 (NP_060365.6)

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
IP Use at 5-10 µg/mg of lysate.

Target

  • Function
    Probable ATP-dependent RNA helicase.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C86129 antibody
    • cb843 antibody
    • DDX27 antibody
    • DDX27_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody
    • DEAD box protein 27 antibody
    • Deficiency of ribosomal subunits protein 1 homolog antibody
    • dJ686N3.1 antibody
    • DKFZp667N057 antibody
    • DRS1 antibody
    • FLJ12917 antibody
    • FLJ20596 antibody
    • FLJ22238 antibody
    • HSPC259 antibody
    • MGC1018 antibody
    • MGC163147 antibody
    • PP3241 antibody
    • Probable ATP-dependent RNA helicase DDX27 antibody
    • RHLP antibody
    • RNA helicase like protein antibody
    • Rrp3p antibody
    see all

Anti-DDX27 antibody images

  • All lanes : Anti-DDX27 antibody (ab85686) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 90 kDa
  • Immunoprecipitation of DDX27 using ab85686 at 10ug/mg of HeLa whole cell lysate.
    20% of immunoprecipitate was loaded par lane, and probed with ab85686 at 1ug/ml (lane 1) or with a control IgG (lane 2).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-DDX27 antibody (ab85686)

ab85686 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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