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Rabbit polyclonal to DDX28
Predicted to work with:
Synthetic peptide corresponding to a region between residue 500 and the C-terminus (residue 540) of human DDX28 (NP_060850.1).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity.
Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.
Belongs to the DEAD box helicase family.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription.
Information by UniProt
DDX 28 antibody
Western blot - Anti-DDX28 antibody (ab70821)
All lanes : Anti-DDX28 antibody (ab70821) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 60 kDa Observed band size: 60 kDa Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-DDX28 antibody (ab70821)
Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.
has not yet been referenced specifically in any publications.
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