Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 254 and 521 of Human DDX3 (UniProt: O00571)

  • Positive control
    • HeLa whole cell, HeLa cytoplasmic or Human embryonic stem cell lysate, Human embryonic stem cells, HeLa cells, Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Images

  • All lanes : Anti-DDX3 antibody (ab151965) at 1/2000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : HeLa cytoplasmic lysate

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 73 kDa
  • Anti-DDX3 antibody (ab151965) at 1/2000 dilution + Human Embryonic stem cell lysate at 20 µg

    Predicted band size : 73 kDa
  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX3 with ab151965 at a 1/250 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed Human embryonic stem cells, labeling DDX3 with ab151965 at a 1/200 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling DDX3 with ab151965 a 1/500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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