Overview

  • Product nameAnti-DDX3 antibody
    See all DDX3 primary antibodies
  • Description
    Rabbit polyclonal to DDX3
  • SpecificityDetects endogenous levels of total DDX3 protein
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non phosphopeptide derived from human DDX3 around the phosphorylation site of threonine 322 (VATPPG).

  • Positive control
    • Human brain tissue (IHC) Extracts from HepG2 cells (WB)

Properties

Applications

Our Abpromise guarantee covers the use of ab61153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Anti-DDX3 antibody images

  • All lanes : Anti-DDX3 antibody (ab61153) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunizing peptide


    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • ab61153 at 1/50 dilution staining human brain tissue without (left) and with (right) blocking peptide; paraffin embedded.
  • ICC/IF image of ab61153 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DDX3 antibody (ab61153)

This product has been referenced in:
  • Ko C  et al. DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level. J Virol 88:13689-98 (2014). Read more (PubMed: 25231298) »

See 1 Publication for this product

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Reduced and denaturing conditions were used in western blot.

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Expiration date: 19-05-2012

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 25 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"