Overview

  • Product nameAnti-DDX3 antibody
    See all DDX3 primary antibodies
  • Description
    Rabbit polyclonal to DDX3
  • Specificityab62581 detects both DDX3 and DBY.
  • Tested applicationsSuitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide from near the center of human DDX3

  • Positive control
    • HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX3/DED1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX3X antibody
    • ATP-dependent RNA helicase DDX3X antibody
    • CAP Rf antibody
    • DBX antibody
    • DDX14 antibody
    • DDX3X antibody
    • DDX3X_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody
    • DEAD box antibody
    • DEAD box protein 3 antibody
    • DEAD box protein 3 X-chromosomal antibody
    • DEAD box X isoform antibody
    • DEAD box, X isoform antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody
    • DEAD/H box 3 antibody
    • DEAD/H box 3, X-linked antibody
    • Helicase like protein 2 antibody
    • Helicase-like protein 2 antibody
    • HLP2 antibody
    • X isoform antibody
    • X-chromosomal antibody
    see all

Anti-DDX3 antibody images

  • ab62581 at 10µg/ml staining DDX3 in HepG2 cells by Immunocytochemistry.
  • Lane 1 : Anti-DDX3 antibody (ab62581) at 1 µg/ml
    Lane 2 : Anti-DDX3 antibody (ab62581) at 2 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-DDX3 antibody (ab62581)

This product has been referenced in:
  • Li Q  et al. DDX3X regulates cell survival and cell cycle during mouse early embryonic development. J Biomed Res 28:282-91 (2014). ICC/IF ; Mouse . Read more (PubMed: 25050112) »
  • Soto-Rifo R  et al. DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. EMBO J 31:3745-56 (2012). WB . Read more (PubMed: 22872150) »

See all 2 Publications for this product

Product Wall

Yes, the antibody can be stored at -20°C, just be sure to avoid multiple freeze-thaw cycles. Frozen is the preferred storage condition beyond a few months.
The X and Y versions are very similar in size. The X isoform has two isoforms of its ow...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"