The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: 1/2500 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceDDX31 is a member of DEAD box proteins family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
The function of this DDX31 has not been determined.
Probable ATP dependent RNA helicase DDX31 antibody
Anti-DDX31 antibody images
Western blot - DDX31 antibody (ab70830)
All lanes : Anti-DDX31 antibody (ab70830) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 95 kDa Observed band size : 95 kDa Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - DDX31 antibody (ab70830)
Detection of DDX31 by Western Blot of Immunprecipitate.
ab70830 at 1µg/ml staining DDX31 in HeLa whole cell lysate immunoprecipitated using ab70830 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.
References for Anti-DDX31 antibody (ab70830)
has not yet been referenced specifically in any publications.
Publishing research using ab70830? Please let us know so that we can cite the reference in this datasheet.
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