SpecificityThis antibody most likely cross-reacts with DDX3X, which shares 91% sequence homology with DDX3Y. This may be why ab72684 shows positive staining in HeLa cells, a female cell line, when DDX3Y is a Y-chromosome linked protein.
FunctionProbable ATP-dependent RNA helicase. May play a role in spermatogenesis.
Tissue specificityTestis-specific. Expressed predominantly in spermatogonia.
Involvement in diseaseNote=DDX3Y is located in the 'azoospermia factor a' (AZFa) region on chromosome Y which is deleted in Sertoli cell-only syndrome. This is an infertility disorder in which no germ cells are visible in seminiferous tubules leading to azoospermia.
Sequence similaritiesBelongs to the DEAD box helicase family. DDX3/DED1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.