Overview

  • Product nameAnti-DDX4 / MVH antibody
    See all DDX4 / MVH primary antibodies
  • Description
    Rabbit polyclonal to DDX4 / MVH
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Human, Pig, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human DDX4/MVH.

    (Peptide available as ab13841.)

  • Positive control
    • This antibody gave a positive signal in Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate and Human Testis Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab13840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with DDX4 / MVH peptide (ab13841).
Flow Cyt Use at an assay dependent concentration. PubMed: 23598447

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/200. PubMed: 19440488
IHC-Fr Use a concentration of 1 µg/ml.
ICC/IF 1/100.
IHC-FoFr 1/400.

Target

  • FunctionProbable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
  • Tissue specificityExpressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX4/VASA subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationCytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDX 4 antibody
    • Ddx4 antibody
    • DDX4_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
    • DEAD box protein 4 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
    • MVH antibody
    • Probable ATP dependent RNA helicase DDX4 antibody
    • Probable ATP-dependent RNA helicase DDX4 antibody
    • VASA antibody
    • Vasa homolog antibody
    see all

Anti-DDX4 / MVH antibody images

  • Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1 µg/ml) overnight at 4 degrees. ab13840 staining is shown in red and Oct4 staining is shown in green. 

    The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.

     

     

     

     

  • ab13840 at 1/1000 staining germ cells of the embryonic mouse testis by ICC/IF.  Cells were PFA fixed and permeabilized in 1% Triton X in PBS prior to blocking in 5% serum for 2 hours at 20°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml

    Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 28 kDa,47 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml

    Lane 1 : Mouse Ovary Lysate at 10 µg
    Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
    Lane 3 : Mouse Ovary Lysate at 10 µg with DDX4 / MVH peptide (ab13841) at 1 µg/ml
    Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg with DDX4 / MVH peptide (ab13841) at 1 µg/ml


    Predicted band size : 76 kDa
    Observed band size : 76 kDa
  • Ab13840 at 1/400 staining mouse fetal testis tissue sections by IHC-P. The tissue was blocked with both BSA and serum and fixed prior to incubation with the antibody for 3 hours at room temperature. A biotinylated secondary antibody was used (Vectastain Elite).

    Please note Bouin's fluid was used as the fixative in this experiment.

    See Abreview

  • ab13840 staining Postnatal Porcine Testis tissue sections by IHC-P.  Bouin's Fixative was used to fix the sections prior to a heat mediated antigen retrieval step in sodium citrate buffer (pH 6).  The sample was blocked in 10% serum for 1 hour at 22°C prior to incubation with the primary antibody, diluted 1/500, for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary

    See Abreview

  • IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-DDX4 / MVH antibody (ab13840)

This product has been referenced in:

See all 105 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Ovary)
Gel Running Conditions Reduced Denaturing (8)
Loading amount 30 µg
Specification Ovary
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jul 21 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Ovary)
Permeabilization No
Specification Ovary
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative Formalin
Username

Abcam user community

Verified customer

Submitted Jun 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Mar 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
Sample Sheep Tissue sections (1-2 month old testis)
Specification 1-2 month old testis
Permeabilization No
Fixative 4%PFA
Username

Abcam user community

Verified customer

Submitted Jan 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/sodium citrate
Sample Mouse Tissue sections (adult ovary)
Specification adult ovary
Permeabilization No
Fixative 4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted Apr 23 2014

Application IHC - Wholemount
Sample Rat Tissue (gonad)
Specification gonad
Username

Abcam user community

Verified customer

Submitted Mar 05 2014

Based on the immunogen sequence used to raise the antibody, it is capable of detecting all 4 isoforms of human DDX4 according to the uniprot file below:

http://www.uniprot.org/uniprot/Q9NQI0

I also ran a blast search using the immunog...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Enzymatic
Sample Rat Tissue sections (Gonad)
Specification Gonad
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 01 2013


Unfortunately this is something that hasnt been tested in our labs so we would not be able to give able to give you a generic answer to this question.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Permeabilization Yes - triton
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2013

1-10 of 41 Abreviews or Q&A

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