Recombinant
RabMAb

Anti-DDX4 / MVH antibody [EPR3849] (ab108392)

Overview

  • Product name
    Anti-DDX4 / MVH antibody [EPR3849]
    See all DDX4 / MVH primary antibodies
  • Description
    Rabbit monoclonal [EPR3849] to DDX4 / MVH
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human DDX4/ MVH

  • Positive control
    • Human testis, Human ovary cancer, K562, seminoma, HeLa, NCCIT and LNCaP cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 79 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Probable ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
    • Tissue specificity
      Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization
      Cytoplasm. Cytoplasm, perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • DDX 4 antibody
      • Ddx4 antibody
      • DDX4_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
      • DEAD box protein 4 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
      • MVH antibody
      • Probable ATP dependent RNA helicase DDX4 antibody
      • Probable ATP-dependent RNA helicase DDX4 antibody
      • VASA antibody
      • Vasa homolog antibody
      see all

    Images

    • All lanes : Anti-DDX4 / MVH antibody [EPR3849] (ab108392) at 1/10000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Seminoma cell lysate
      Lane 5 : HeLa cell lysate
      Lane 6 : NCCIT cell lysate
      Lane 7 : LNCaP cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 79 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

    References

    ab108392 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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