Anti-DDX42 antibody (ab89075)
Key features and details
- Mouse polyclonal to DDX42
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DDX42 antibody -
Description
Mouse polyclonal to DDX42 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human DDX42 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86XP3-2 -
Positive control
- A431 cell lysate. Cell lysate from transfected 293T cells. HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.
|
Notes |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. |
Target
-
Relevance
This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. -
Cellular localization
Cytoplasm. Nucleus speckle. Nucleus> Cajal body. Note: Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles. -
Database links
- Entrez Gene: 11325 Human
- SwissProt: Q86XP3 Human
-
Alternative names
- ATP-dependent RNA helicase DDX42 antibody
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 antibody
- DEAD box polypeptide 42 protein antibody
see all
Images
-
Anti-DDX42 antibody (ab89075) at 1 µg/ml + A431 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted? -
All lanes : Anti-DDX42 antibody (ab89075) at 1 µg/ml
Lane 1 : cell lysate from DDX42 transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted? -
ab89075 at 10 µg/ml staining DDX42 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab89075 has been referenced in 1 publication.
- Sun L et al. In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Cell 184:1865-1883.e20 (2021). PubMed: 33636127