Overview

  • Product nameAnti-DDX42 antibody
    See all DDX42 primary antibodies
  • Description
    Mouse polyclonal to DDX42
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human DDX42 aa 1-819.
    Sequence:

    MAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPT AGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYN EHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIR KSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQK ELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQ AKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEY QVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEA NEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANN LKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSI KTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNL EGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLG SENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLS NQ KAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPG FGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRG SSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHG GSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSK TDKTADGFAVPEPPKRKKSRWDS


    Database link: Q86XP3-2

  • Positive control
    • A431 cell lysate. Cell lysate from transfected 293T cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.

Target

  • RelevanceThis gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene.
  • Cellular localizationCytoplasm. Nucleus speckle. Nucleus> Cajal body. Note: Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.
  • Database links
  • Alternative names
    • ATP-dependent RNA helicase DDX42 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 antibody
    • DEAD box polypeptide 42 protein antibody
    • DEAD box protein 42 antibody
    • EC 3.6.1.- antibody
    • FLJ43179 antibody
    • RHELP antibody
    • RNA helicase-like protein antibody
    • RNA helicase-related protein antibody
    • RNAHP antibody
    • SF3b DEAD-box protein antibody
    • SF3b125 antibody
    • SF3b125 DEAD-box protein antibody
    • Splicing factor 3B-associated 125 kDa protein antibody
    see all

Anti-DDX42 antibody images

  • Anti-DDX42 antibody (ab89075) at 1 µg/ml + A431 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : ~130 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-DDX42 antibody (ab89075) at 1 µg/ml

    Lane 1 : cell lysate from DDX42 transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 103 kDa
    Observed band size : ~130 kDa (why is the actual band size different from the predicted?)
  • ab89075 at 10 µg/ml staining DDX42 in paraformaldehyde-fixed, permeabilised HeLa cells by immunofluorescence.

References for Anti-DDX42 antibody (ab89075)

ab89075 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"