Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX43
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-235 of Human DDX43.

  • Positive control
    • NT2D1 whole cell lysate. Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Tissue specificity
    Expressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.
  • Sequence similarities
    Belongs to the DEAD box helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 KH domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 13 antibody
    • CT13 antibody
    • DDX43 antibody
    • DDX43_HUMAN antibody
    • DEAD box protein 43 antibody
    • DEAD box protein HAGE antibody
    • HAGE antibody
    • Helical antigen antibody
    • Probable ATP dependent RNA helicase DDX43 antibody
    • Probable ATP-dependent RNA helicase DDX43 antibody
    see all

Images

  • Anti-DDX43 antibody (ab154634) at 1/2000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labelling DDX43 with ab154634 at a 1/500 dilution.

References

ab154634 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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