Overview

  • Product nameAnti-DDX43 antibody
    See all DDX43 primary antibodies
  • Description
    Mouse monoclonal to DDX43
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: SHHGGAPKAS TWVVASRRSS TVSRAPERRP AEELNRTGPE GYSVGRGGRW RGTSRPPEAV AAGHEELPLC FALKSHFVGA VIGRGGSKI, corresponding to amino acids 2-90 of Human DDX43 (NP_061135), with a proprietary tag.

  • Positive control
    • DDX43-transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Tissue specificityExpressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.
  • Sequence similaritiesBelongs to the DEAD box helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 KH domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 13 antibody
    • CT13 antibody
    • DDX43 antibody
    • DDX43_HUMAN antibody
    • DEAD box protein 43 antibody
    • DEAD box protein HAGE antibody
    • HAGE antibody
    • Helical antigen antibody
    • Probable ATP dependent RNA helicase DDX43 antibody
    • Probable ATP-dependent RNA helicase DDX43 antibody
    see all

Anti-DDX43 antibody images

  • All lanes : Anti-DDX43 antibody (ab77416) at 5 µg/ml

    Lane 1 : DDX43-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • Anti-DDX43 antibody (ab77416) at 5 µg/ml + immunogen (tagged 88 aa recombinant fragment) at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-DDX43 antibody (ab77416)

ab77416 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"