Overview

  • Product nameAnti-DDX43 antibody
    See all DDX43 primary antibodies
  • Description
    Rabbit polyclonal to DDX43
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within the amino acids 125-175 (KAVIDNFVKK LEENYNSECG IDTAFQPSVG KDGSTDNNVV AGDRPLIDWD Q) of Human DDX43, Swiss Prot Q9NXZ2.

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 73 kDa).
IP Use at 10 µg/mg of lysate.

Target

  • Tissue specificityExpressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.
  • Sequence similaritiesBelongs to the DEAD box helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 KH domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 13 antibody
    • CT13 antibody
    • DDX43 antibody
    • DDX43_HUMAN antibody
    • DEAD box protein 43 antibody
    • DEAD box protein HAGE antibody
    • HAGE antibody
    • Helical antigen antibody
    • Probable ATP dependent RNA helicase DDX43 antibody
    • Probable ATP-dependent RNA helicase DDX43 antibody
    see all

Anti-DDX43 antibody images

  • All lanes : Anti-DDX43 antibody (ab86596) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 73 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Immunoprecipitation/ Western Blot of ab86596
    Lane 1: ab86596 at 10µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab86596 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.

References for Anti-DDX43 antibody (ab86596)

This product has been referenced in:
  • Ambrosini G  et al. Overexpression of DDX43 mediates MEK inhibitor resistance through RAS Upregulation in uveal melanoma cells. Mol Cancer Ther 13:2073-80 (2014). Read more (PubMed: 24899684) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"