Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling DDX46 with ab72083 at 1/200 (1µg/ml). Detection: DAB.
Western blot - DDX46 antibody (ab72083)
All lanes : Anti-DDX46 antibody (ab72083) at 0.04 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg Lane 2 : Whole cell
lysate from HeLa cells at 15 µg Lane 3 : Whole cell
lysate from HeLa cells at 5 µg Lane 4 : Whole cell
lysate from 293T cells at 50 µg Lane 5 : Whole cell
lysate from NIH 3T3 cells at 50 µg
Analysis of Human DDX46 by Immunoprecipitation in whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab72083 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). ab72083 was used for WB detection at 1 µg/ml. Detection: chemioluminescence, exposure time 3 seconds.