Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX47
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DDX47 aa 1-50 (N terminal). The exact sequence is proprietary.
    Sequence:

    MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI


    Database link: Q9H0S4

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176852 was affinity purified using an epitope specific to DDX47 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.

Target

  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDX47 antibody
    • DDX47_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 47 antibody
    • DEAD box helicase 47 antibody
    • DEAD box protein 47 antibody
    • DKFZp564O176 antibody
    • E4 DBP antibody
    • FLJ30012 antibody
    • HGNC:18682 antibody
    • HQ0256 antibody
    • MSTP162 antibody
    • Probable ATP dependent RNA helicase DDX47 antibody
    • Probable ATP-dependent RNA helicase DDX47 antibody
    see all

Images

  • All lanes : Anti-DDX47 antibody (ab176852) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa


    Exposure time : 3 minutes
  • Ab176852 immunoprecipitating DDX47 in HeLa whole cell lysate. 3µg/mg of cell lysate was incubated with primary antibody. 

References

ab176852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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